Phosphate Buffered Saline Recipe 10X For Lab Use

Phosphate Buffered Saline (PBS) is a versatile buffer solution commonly used in biological research. It’s essential for maintaining the pH and osmolarity of tissues or cell cultures, which is crucial for experiments and applications in biochemistry, molecular biology, and more. Below, you’ll find a detailed guide on how to prepare a 1X solution of PBS, along with the necessary ingredients and step-by-step instructions.

Image of PBS Preparation

This image illustrates the preparation of PBS. It’s important to use quality materials and follow the correct procedures to ensure the solution’s effectiveness.

Ingredients for 1X PBS

To prepare a standard 1X Phosphate Buffered Saline solution, you will need the following ingredients:

8 g of Sodium chloride (NaCl)
0.2 g of Potassium chloride (KCl)
1.44 g of Disodium hydrogen phosphate (Na2HPO4)
0.24 g of Monopotassium phosphate (KH2PO4)
Distilled or deionized water (up to 1 liter)

Instructions for Preparing PBS

Follow these steps to prepare your 1X PBS solution:

Start by measuring out the required amounts of Sodium chloride, Potassium chloride, Disodium hydrogen phosphate, and Monopotassium phosphate using a scale for accuracy.
In a clean beaker or container, combine the solid ingredients. Mix them gently to ensure even distribution of the components.
Slowly add distilled or deionized water to the mixture while stirring. This helps to dissolve the salts completely and creates a homogeneous solution.
Once all solids are completely dissolved, transfer the mixture to a 1-liter volumetric flask or graduated cylinder for precise measurement.
Add distilled or deionized water to bring the total volume to exactly 1 liter. This step is critical to maintaining the correct concentrations of salts in the solution.
Mix the solution well. It is recommended to check the pH of the PBS solution. The ideal pH for PBS is around 7.2 to 7.4. If adjustment is needed, you can use hydrochloric acid (HCl) or sodium hydroxide (NaOH) to achieve the desired pH.
Finally, label the solution appropriately and store it in a clean bottle. PBS can be kept at 4°C for short-term use, but it’s best to prepare fresh solutions for long-term storage.

There you have it! A simple and effective method for preparing your very own Phosphate Buffered Saline solution. This buffer will be an invaluable addition to your laboratory preparations and experiments.